<i>Alteromonas macleodii</i> KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism
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منابع مشابه
Streptomycin resistance in a streptomycin-producing microorganism.
Cell-free extracts of Streptomyces bikiniensis contain an adenosine 5'-triphosphate-dependent kinase which inactivates streptomycin (Sm) and dihydrostreptomycin by phosphorylation. The products have been identified as streptomycin 6-phosphate and dihydrostreptomycin 6-phosphate. Activity was not present in logarithmic-phase cells, which were susceptible to 25 mug of Sm per ml. In stationary-pha...
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κ-Carrageenase belongs to glycoside hydrolase family 16 and cleaves the β-(1→4) linkages of κ-carrageenan. In this study, genes encoding the full-length (cgkZ), Por secretion tail-truncated (cgkZΔPst) and carbohydrate binding domain-truncated (cgkZΔCBM) κ-carrageenase proteins were expressed in Pichia pastoris. The copy numbers of gene cgkZ, cgkZΔPst and cgkZΔCBM were 7, 7 and 6, respectively. ...
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Effective degradation of κ-carrageenan by isolated Thalassospira sp. fjfst-332 is reported for the first time in this paper. It was identified by 16S rDNA sequencing and morphological observation using Transmission Electron Microscopy (TEM). Based on a Plackett-Burman design for significant variables, Box-Behnken experimental design and response surface methodology were used to optimize the cul...
متن کاملCharacterization of a κ-Carrageenase from Marine Cellulophaga lytica strain N5-2 and Analysis of Its Degradation Products
A carrageenan-degrading marine Cellulophaga lytica strain N5-2 was isolated from the sediment of carrageenan production base. A κ-carrageenase (EC 3.2.1.83) with high activity was purified to electrophoretic homogeneity from the culture supernatant by a procedure of ammonium sulfate precipitation, dialyzing and gel filtration on SephadexG-200 and SephadexG-75. The purified enzyme was verified a...
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Previously, we reported a series of 16-membered macrolide antibiotics, designated izenamicins, produced by Micromonospora sp. YS-02930K1}. In the course of screening for intermediates of izenamicins, we obtained five 16-memberedlactone compounds and examined the biosynthetic pathways of izenamicins B2 and B3 after lactone formation. In this paper we describe the isolation, characterization and ...
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ژورنال
عنوان ژورنال: International Journal of Microbiology and Biotechnology
سال: 2019
ISSN: 2578-9678
DOI: 10.11648/j.ijmb.20190404.13